HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 00. The SpectraMax iD5 and SpectraMax i3x readers use SoftMax® Pro Data Acquisition and Analysis Software. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). S. Italic and boldface type are used for emphasis. Enzyme titration and time course, cofactor and substrate Km,andIC50 for known inhibitors are demonstrated. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Both instruments demonstrated good performance for the assay. Features. The following document is intended. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMulti-mode Microplate Readers. 2. The SpectraMax M2 and M2e are versatile and reliable. The dual monochromator optics allow. The SpectraMax M5e reader offers the additional benefit of being certified . Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. 4, Z’ = 0. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . 4. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The CellTiter-Glo® 2. In this application note we show how the SpectraMax ® i3, SpectraMax ® Paradigm ®, and SpectraMax ® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF ® assays with excellent Z’ factors and highly reproducible EC 50 values. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. 1) SpectraMax Glo Steady Luc Reporter Assay (6. Spectranet customer portalBioTek Cytation™ 5 Cell Imaging Multi-Mode Reader. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes Generation of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). A fluorescence microscope with high-throughput screening capabilities (Axio Observer. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. SpectraMax Mini: 17. Sunnyvale, Calif. Homogeneous Time-Resolved Fluorescence (HTRF) . KEY FEATURESMolecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 0200-0729. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 00 USD. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. Thermo Lab Systems Multiskan Ascent 354 $1,400. SpectraMax Glo and DuoLuc. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 6 x 10-15. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax Plus384 SpectraMax 340PC384 SpectraMax 190. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Five preferred detection modes. 2 only) SpectraMax M-series Microplate readers. Absorbance at a wavelength of 450 nm was measured using the SpectraMax M5e multi-mode microplate reader (Molecular Devices, San Jose, CA, USA) to quantify cytokine. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. We also buy, refurbish, and resell used lab equipment. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. 2 x 10-14. No disponible por el momento. For more information about the SpectraMax® M5e Multi-Mode Microplate Reader or other products from Molecular Devices, please visit our website (or send an email to. SPECTRAMAX XS XENON REPLACEMENT BULB FOR MOLECULAR DEVICES SPECTRAMAX XPS XENON, SPECTRAMAX XS. SpectraMax Mini: 17. We would like to show you a description here but the site won’t allow us. The large analog PMT works with monochromators and is used during fluorescence. for Cisbio Bioassays HTRF® technology. com 10. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. In the M2, M5 and Plus 384, the light. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the. NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Molecular Devices SpectraMax™ M5 Microplate Reader w/ Warranty (Svc Contract Available) $29,500. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. 1. 0 17. 005) Photometric Precision多功能酶标仪SpectraMax M5e. Each tube of fluorophore also contained ROX. Find regional support. 316 grating motor driver faulty. The measurements were performed in triplicate. Status: Reviewer Member since: 2020 Organization: IIUM Ease of use After sales service Value for money Great results, easy life, bright future. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seMolecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available) $34,000. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. D 1 1. 1. It was also read on the monochromator-based SpectraMax® M5e Multi-Mode Microplate Reader using optimized wavelengths. Dual monochromators for assay flexibility With. 1. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. In the M2, M5 and Plus. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Add to cart. 1. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Ferguson, Ph. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. Other technologies such as imaging. 2 x 10-14. SpectraMax Mini: 17. The donor and acceptor can be used to label a variety of biomolecules, for. 1(a). 00 USD. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Description 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. book Page 1 Tuesday,. 314 low lamp level warning. 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 8. 2. elegans, and Alcanivorax borkumensis. The optical systemsThe BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Lector de microplacas M5E-SC SpectraMax M5e con contrato de servicio de 1 año - Lector de placas multimodo SpectraMax M5/M5e, dispositivos moleculares - cada uno . Add to Cart. Examples: “Press carefully to engage,” “Do not. , 19 May 2011. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. . ZERO BIAS - scores, article reviews, protocol conditions and moreThe NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. The following document is intended to demonstrate setup of this instrument. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. KeySpan adapter for SoftMax Pro and SpectraMax readers. 4. 1. FlexStation II. book Page 21 Wednesday, July 7, 2010 2:09 PM. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Detection modes include: • Endpoint, kinetic, spectral and well-scanning • Comprehensive data analysis • Robotics compatibility • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. SpectraMax M5e: 118. WARNING! The instrument weighs approximately 36 pounds. It was difficult and time consuming to undertake quality, repetitive research. High sensitivity and. Danaher Inc spectramax m5 m5e fluorescent plate reader Spectramax M5 M5e Fluorescent Plate Reader, supplied by Danaher Inc, used in various techniques. Table of Contents. M3,M4,M5,M5e,FlexStation 3 0200-6186 SpectraTest LM1 Luminescence ValidationPlate SpectraMaxiD3,iD5,i3x,i3,M3,M4,M5,M5e,SpectraMaxL, FlexStation 3 0200-2420Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s. SpectraMax Quant dsDNA Assay Kits. Path Check Pathlength Measurement Multimode Reader Comparison Chart. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 306 plate air cal fail,low light. 313 reference gain check fail. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Page 75. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 1. 305 reference level saturation. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Explore PDF for more details. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. SpectraMax M2. The assay was set up in a low-volume 384- well black plate using the pipetting. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. com A. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 1. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The analysis capabilities of the software provide automated quantitation in an easy-to-read, user-customizable report format. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. GFP fluorescence was read on days 1, 4 and 7, using a SpectraMax M5e Microplate Reader (Molecular Devices). 5 mP standard deviation at 1 nMMolecular Devices SpectraMax M5e Microplate Reader $26,500. A two-fold dilution series of JQ1 (in DMSO) starting from 1 μM was prepared. Both instruments demonstrated good performance for the assay. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. 00 USD. Products : SpectraMax M3 reader, SoftMax Pro Software, SpectraMax M5/M5e reader, SpectraMax L reader, SpectraMax M4 reader Is the Gaussia Luciferase (GLuc) Assay from New England Biolabs compatible with my plate reader? The information for the GLuc Assay Kit (Cat no. Read mode. KEY FEATURES Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Multimode Reader Comparison Chart. 72 MB. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. 0. e. 485. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. #M5e) Methods Microplate reader compatibility test. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. the performance of the Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader with an integrated 8-channel or 16-channel pipettor into one compact benchtop reader. M3-M5e_manual. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. AquaMax® 4000. 1. Settings are for each instrument are shown in Table 1. 3. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. SpectraMax® Aquamax. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. HEK293 cells in growth medium (MEM/10% FBS/1% penicillin/streptomycin) were seeded at 50,000 cells per well, 50 μL per well, in a 96-well poly-L-lysine-coated microplate. SpectraMax® ABS. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. Plate chamber design of the SpectraMax category 1 microplate readers. Description 1. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAn example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. HTRF is compatible with a large range of available microplate readers. This document is provided to customers who have purchased Molecular Devices, Inc. Features. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter. These robust readers have been placed in labs from Antarctica to the. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. The drawing shows a standard microplate of 15 mm height covered with a lid. 00We would like to show you a description here but the site won’t allow us. SpectraMax Mini. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Open the SoftMax Pro Software and select TR-FRET for the Read Mode and Endpoint for the Read Type. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 5, Z’ = 0. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Call 516-939-0888 to check current. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. Gemini EM. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 8 x 10-15. 8. Molecular Devices. 3. Dual monochromators for assay flexibility. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Cell monolayers were decalcified with 0. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. e. SpectraMax Injector Cartridge with SmartInject™ Technology. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. D 47 7. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 72 MB. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. 5. Anne T. 1. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. The x and y calibrations are the same 60 µm per pixel 2. You must scan the fluorochrome of interest in the SpectraMax M5 or M5e reader to determine the optimal excitation and emission wavelengths for your application. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. Please see the exact video and photos below. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. -- JULY 19, 2016 Cables. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. FlexStation™ II. After The Plate Is Read, Data Will Appear In The Plate Section: 11. Microplate Readers include the M3, M4,. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode. SpectraMax M5e: 118. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Molecular Devices SpectraMax® M5/M5e Microplate Reader Have A Question? Contact Our Technical Support Team NA: 800-955-6288 Or INTL: 760-603-7200 Ext. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 1(a). The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 6 x 10-15. (PRWEB) July 19, 2016 -- Molecular Devices, LLC, a leader in protein and cell biology technologies, today announced the launch of the SpectraMax. 1. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). Lift up the lid over the chamber to insert or remove a cuvette. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. Download PDF. Page 75. 00 USD. . SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 1. SpectraMax Paradigm. E3300S/L) can be found on the New. Measured on the SpectraMax M5e™ reader, AcGFP (jellyfish), ZsGreen (reef coral), DsRed (reef coral) fluorescent proteins expressing cells can be assayed on a single plate, down to approximately 30 cells per well (384-well format), highlighting the increased light throughput efficiency and sensitivity of the MDS Analytical Technologies. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 3. D 1 1. 4, Z’ = 0. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Venue Address. 21 MB. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 1. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3? The M3/M4/M5/M5e/Flex3 multi-mode readers have a higher instrument noise level floor as compared to the dedicated luminometers. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. The optical systemsThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe SpectraMax M5e reader offers the additional benefit of being certified . Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. 1, left panel) and individual wells (Fig. STAKMAX. C. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Endpoint, kinetic, spectrum. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. SpectraMax Gemini EM/XPS. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. View the Molecular Devices website. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. Description 1. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). Note: In this user guide, all references to SpectraMax Multi-Mode. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. book Page 1 Wednesday, July 7, 2010 2:09 PM. FlexStation™ III. Meso Scale Discovery 1200 Sector Imager 2400. After excluding the background values, the cross‐reactivity of the. Introduction. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. Choose cables that meet the requirement described in this appendix. Introduction. Jun 9th, 2023. Listing SKU: DIS-58825-molecular-devices-spectramax-m5e-microplate-readerIn May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader in space. SpectraMax L: 36. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and other niche laboratory equipment lines. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. 8 x 10-15. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. The dual monochromator optics allow. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax M5e. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionSpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Qualify the fluorescence performance of the SpectraMax . Molecular Devices. View the Molecular Devices website. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. In particular, these parameters should be entered as defined in the table below. 5, Z’ = 0. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). SpectraMax ABS Plus SpectraTest ABS2 v1. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change.